Gill transcriptome response to changes in environmental calcium in the green spotted puffer fish

first_imgBackground Calcium ion is tightly regulated in body fluids and for euryhaline fish, which are exposed to rapid changes in environmental [Ca2+], homeostasis is especially challenging. The gill is the main organ of active calcium uptake and therefore plays a crucial role in the maintenance of calcium ion homeostasis. To study the molecular basis of the short-term responses to changing calcium availability, the whole gill transcriptome obtained by Super Serial Analysis of Gene Expression (SuperSAGE) of the euryhaline teleost green spotted puffer fish, Tetraodon nigroviridis, exposed to water with altered [Ca2+] was analysed.Results Transfer of T. nigroviridis from 10 ppt water salinity containing 2.9 mM Ca2+ to high (10 mM Ca2+ ) and low (0.01 mM Ca2+) calcium water of similar salinity for 2-12 h resulted in 1,339 differentially expressed SuperSAGE tags (26-bp transcript identifiers) in gills. Of these 869 tags (65%) were mapped to T. nigroviridis cDNAs or genomic DNA and 497 (57%) were assigned to known proteins. Thirteen percent of the genes matched multiple tags indicating alternative RNA transcripts. The main enriched gene ontology groups belong to Ca2+ signaling/homeostasis but also muscle contraction, cytoskeleton, energy production/homeostasis and tissue remodeling. K-means clustering identified co-expressed transcripts with distinct patterns in response to water [Ca2+] and exposure time.Conclusions The generated transcript expression patterns provide a framework of novel water calcium-responsive genes in the gill during the initial response after transfer to different [Ca2+]. This molecular response entails initial perception of alterations, activation of signaling networks and effectors and suggests active remodeling of cytoskeletal proteins during the initial acclimation process. Genes related to energy production and energy homeostasis are also up-regulated, probably reflecting the increased energetic needs of the acclimation response. This study is the first genome-wide transcriptome analysis of fish gills and is an important resource for future research on the short-term mechanisms involved in the gill acclimation responses to environmental Ca2+ changes and osmoregulation.last_img read more

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Whale Flippers Inspire Aeronautical Engineers

first_imgHave you seen the bumpy flippers on humpback whales, you know, the species whose males serenade their mates?  Don’t laugh.  Scientists have found that the ungainly flippers actually have superior lift, less drag, and are less susceptible to stalling.  Engineers are imitating the whale flippers for advanced aircraft and helicopter rotors, reports EurekAlert from studies at Duke University.  “Humpback whales maneuver in the water with surprising agility for 44-foot animals,” says the press release.  Test wings modeled after the flipper had 8% better lift, 32% lower drag, and could withstand stalling at a 40% steeper wing angle.We don’t need to design so many things from scratch.  Biomimetics is a modern application of an ancient principle expressed by Job, “ask now the beasts, and they shall teach thee” (Job 12:7).(Visited 7 times, 1 visits today)FacebookTwitterPinterestSave分享0last_img read more

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Ohio AgriBusiness Association Annual Conference

first_imgShare Facebook Twitter Google + LinkedIn Pinterest The Ohio AgriBusiness Association will hold its annual Industry Conference Feb. 3-4, 2016 at the Columbus Marriott Northwest, providing education and networking opportunities for agribusiness professionals, including the OABA Industry Dinner and OABA Annual Meeting.This will be the fourth year the Industry Conference has been held, with more than 300 industry professionals attending the 2015 event.“The OABA Industry Conference continues to be an exceptional event that provides agribusiness professionals with access to experts on current agricultural issues in Ohio,” said Chris Henney, OABA president and CEO. “This will be a valuable experience for attendees, with many opportunities to gain insight and perspectives to help tackle the challenges and opportunities that lie ahead.”Widely-respected speakers and industry professionals will share their expertise during the two-day conference. Topics include:Nutrient stewardshipCustom applicationInsect controlNitrogen managementUpdates from Ohio State University Extension, the Environmental Protection Agency and the Occupational Safety and Health Administration.General session speakers include Chris Reynolds with PotashCorp, Kathy Mathers with The Fertilizer Institute, Marty Ruikka with ProExporter and Kim Chesky with LeadingWise.This year’s keynote speaker will be retired Army Col. Dr. Peter Mansoor, presenting on “The Rise of ISIS.” Mansoor’s 26-year career in the military included two combat tours and serving as executive officer to retired Army Gen. David Petraeus in Iraq.A complete agenda is available at www.oaba.net/events_conference.Continuing education units (CEUs) are available for both Certified Crop Advisers and state licensed pesticide applicators that attend the conference.All-inclusive registration for the event includes admission to both days of educational programming and all meals, including the Industry Networking Reception & Dinner and OABA Annual Meeting. Registration fees are as follows:Member: $260 ($335 after Jan. 8, 2016)Non-Member: $400 ($475 after Jan. 8, 2016)Individual day and reception/dinner-only registrations are also available, as are exhibitor and sponsorship opportunities. Visit www.oaba.net/events_conference for more information and to register. All cancellations must be made by Jan. 22, 2016 to be eligible for a refund.Hotel accommodations for the Columbus Marriott Northwest can be made online by visiting www.oaba.net/events_conference or by calling 614-791-1000 and using the code “Ohio AgriBusiness Association.” The deadline to make reservations is Jan. 22, 2016.last_img read more

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Poll: Is OpenStack Now More Attractive Following the Amazon Web Services Outage?

first_imgTop Reasons to Go With Managed WordPress Hosting Why Tech Companies Need Simpler Terms of Servic… A Web Developer’s New Best Friend is the AI Wai… The OpenStack Conference and Design Summit is happening this week. The group assembling represents a community that in less than a year has attracted a worldwide developer group and enough influence to attract organizations such as Canonical, Citrix and Cisco. These are partners which are now contributing to the effort to build an infrastructure that anyone can use to build their own cloud environment.OpenStack is also a contrast to Amazon Web Services (AWS), which had such an outage last week that people are starting to see what the alternatives are to the service, which is now the dominant leader in the space.James Staten of Forrester wrote in a blog post today that he has been fielding calls from AWS customers that have lost data, following the AWS outage. These are customers who are now open to new alternatives. For OpenStack that means it has more of a wedge than it did before. It highlights a few things. Not just that OpenStack is an open infrastructure compared to AWS. It also points to the differences in the ecosystems.Staten:Amazon’s massive ecosystem of management software, SaaS, Platform as a Service (PaaS) and independent software vendor (ISV) partners are all just customers of AWS. They aren’t contributors, nor are they I&O peers. OpenStack’s ecosystem includes many of the above but also service providers, government agencies and enterprises who are implementing OpenStack as well. Which means that if you choose to implement OpenStack for your private cloud or you are a hoster implementing it for your IaaS, there is an I&O peer network you can call on in times of crisis who have intimate knowledge of the software and can help you diagnose the problem and even potentially supply a patch to reverse the problem.OpenStack is still a new entity. But its momentum is unquestioned. And it may have a better chance at succeeding, following the unfortunate series of events that fell upon AWS and its customers last week.What do you think?ddy.com/features-surveys/”>Market Research Related Posts center_img alex williams Tags:#cloud#cloud computing#news 8 Best WordPress Hosting Solutions on the Marketlast_img read more

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San Miguel overcomes Globalport behind Santos’ heroics

first_imgView comments Don’t miss out on the latest news and information. “Even though the players played sluggish, we were able to to come out as winners and I’m happy for them,” said San Miguel head coach Leo Austria as they improved to 3-1.Despite taking a 112-104 lead with 2:12 remaining in the game, the Beermen weren’t able to easily shake off the Batang Pier as the latter pounded to make it a one-possession game, 114-112, on the nine-second mark.FEATURED STORIESSPORTSWATCH: Drones light up sky in final leg of SEA Games torch runSPORTSSEA Games: Philippines picks up 1st win in men’s water poloSPORTSMalditas save PH from shutoutCompounding San Miguel’s situation was June Mar Fajardo’s injury.The three-time MVP is still nursing his injured right calf he sustained before the Fiba Asia Championship. LATEST STORIES Photo by Tristan Tamayo/ INQUIRER.netSan Miguel bounced back and sank Globalport, 115-112, to get its third win in the PBA Governors’ Cup Friday at Smart Araneta Coliseum.The Beermen, who are coming off a three-week break, had to rely on late-game heroics from Arwind Santos, who made the game’s final free throw and disrupted Murphy Holloway’s potential-game tying shot.ADVERTISEMENT SEA Games in Calabarzon safe, secure – Solcom chief Brace for potentially devastating typhoon approaching PH – NDRRMC UPLB exempted from SEA Games class suspension SEA Games: PH’s Alisson Perticheto tops ice skating short program Catriona Gray spends Thanksgiving by preparing meals for people with illnessescenter_img LIST: Class, gov’t work suspensions during 30th SEA Games Read Next MOST READ “I was not able to give June Mar a lot of minutes because of his injury and he wasn’t able to join practice for the past two days because he’s still recuperating,” said Austria.Still, San Miguel enjoyed a three-pronged attack with Wendell McKines doing the most damage with 35 points, 14 rebounds, and seven assists.Santos added 30 points to go along eight boards and five blocks while Alex Cabagnot had 27 points, seven rebounds, and four assists.Terrence Romeo led Globalport with 26 points and six boards while Holloway added 25 points and 23 boards.ADVERTISEMENT Trending Articles PLAY LIST 00:50Trending Articles01:30’Excited’ Terrence Romeo out to cherish first PBA finals appearance00:59Sports venues to be ready in time for SEA Games01:37Protesters burn down Iran consulate in Najaf01:47Panelo casts doubts on Robredo’s drug war ‘discoveries’01:29Police teams find crossbows, bows in HK university01:35Panelo suggests discounted SEA Games tickets for students02:49Robredo: True leaders perform well despite having ‘uninspiring’ boss02:42PH underwater hockey team aims to make waves in SEA Games LOOK: Venues for 2019 SEA Games WATCH: Streetboys show off slick dance moves in Vhong Navarro’s wedding Bench-clearing brawl erupts in La Salle, FEU pre-season gamelast_img read more

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